Atcc aav2 plasmid. Bioz Stars score: 95/100, based on 1 PubMed citations.
Atcc aav2 plasmid This strain was developed by the Adeno-associated Virus Reference Standard Working Group (AAVRSWG). Moullier, INSERM UMR649 Nantes, France), a concentrated adenovirus helper virus (the adenovirus type 5 reference standard material, ARM) (VR-1516; ATCC) for the transduction and infectivity assays, and ATCC Plasmid Type Unspecified Cloning Method Unknown Size 8700 Notes Contains the complete viral genome for use in site-specific mutagenesis studies. Aug 18, 2023 · For example, AAV2/5 targets neurons that are not efficiently transduced by AAV2/2, and is distributed more widely in the brain, indicating improved transduction efficiency. 3. Upon receipt from ATCC the adenovirus should be thawed on ice, immediately aliquoted (25ul) and snap frozen on dry ice/ ethanol. AAV2 is one of the most well-studied and widely used serotypes of adeno-associated virus. The kit contains an optimized mix of the three plasmids, as well as a separate tube Mar 29, 2025 · The pRCap-AAV2 plasmid is compatible with our AAVdual and AAVtri systems, supplying AAV2 Rep and AAV2 Cap functions. The pDP8 helper plasmid harbors the rep gene from AAV2, the cap gene from AAV8, the adenovirus helper genes E2A, E4, VA-RNA and the ampicillin resistance gene. Adeno-associated viral (AAV) vectors offer precise delivery of genetic material across a wide range of tissue types with minimal immune response in vivo. This strain depends on the helper virus Human adenovirus 2 (ATCC VR-846) for replication. Internal standard material calibrated with the ATCC reference standard material for AAV2 (VR-1616) was used for the final titer declaration. designed a qPCR method for AAV titration that targeted a 62-nucleotide (nt) sequence of the AAV2 stem region of the ITRs with a limit of detection (LOD) of 50 copies. pD8 is available through Plasmidfactory (PlasmidFactory GmbH & Co, Biefield, Germany) as a product number PF478. Bioz Stars score: 95/100, based on 1 PubMed citations. 1. 0 plus pAAVtri-GOI, it enables the packaging of AAV2 viruses carrying the gene of interest (GOI). ATCC hereby represents and warrants that the material provided under this certificate has been subjected to the tests and procedures specified and that the results described, along with any other data provided in this certificate, are true and correct to the best of the company’s knowledge and belief. 2. They have become an essential tool for studying gene function, modeling diseases, and advancing gene therapy development. These three plasmids must be used with a HEK293 cell line that stably expresses the adenovirus E1 gene. This reference is produced using the AAVone system with the pAAVone-AAV2-CMV-EGFP-2. . Transfection into human 293 cells with an Ad helper virus results in infectious AAV virus particles. In order to produce recombinant AAV vectors, researchers need to use three plasmids: A transfer plasmid (or cis plasmid The Gibco AAV-MAX Control Plasmids Kit consists of an optimized mix of three plasmids needed to produce adeno-associated virus serotype 2 (AAV2)−expressing GFP: pAAV-CMV-emGFP, pAAV-Rep2Cap2, and pHelper. 25 For the SV40 polyA qPCR assay, a linearized pTR-UF11 plasmid 26 (ATCC, MBA- 331) was used to generate a standard curve. coli; and ATCC® CRL-2972TM HeLa RC32 Cell Line The AAV2 reference-2. pAV1 (ATCC 37215) and pAV2 (ATCC 37216) both contain the complete AAV2 genome, but in opposite Recombinant Aav2 Reference Standard Material, supplied by ATCC, used in various techniques. AAV2 reference material (ATCC # VR1616) Aliquots obtained from ATCC should be directly stored at -80°C and thawed only before use for this assay. SKU: SR000000-AAV2 AAV Serotype: AAV2 Plasmid Size: 7,497 bp Bacterial Resistance(s) : Amp Storage: -20°C The lot-specific titers were assigned using AAV2 ELISA (PRAAV2R and PRAAV2XP) and PCR using eGFP primers. 8kb plasmid. When co-transfected with either: pAAVdual-GOI plasmids, or mini-pHelper-1. It has been a workhorse for gene therapy The AAV2 plasmid, psub201, containing the AAV-2 ITRs was used to generate standard curves for the ITR qPCR assay. pAV1 (ATCC 37215) and pAV2 (ATCC 37216) both contain the complete AAV2 genome, but in opposite orientations. It is not intended New Viral Gene Vector Reference Material, Plasmid and Cell Line Now Available ATCC® VR-1616TM Recombinant Adeno-Associated Virus, Type 2, Reference Material (rAAV2-RSS); ATCC® MBA-331TM pTR-UF-11 Plasmid in E. 2. 61 In studies evaluating methods for quantifying genomes extracted from purified AAV virions following removal of plasmid DNA, factors such as assay design and ATCC® Catalog No. The reagents, which are now available from the ATCC, include a cell line expressing the AAV2 rep and cap genes (HeLa32; kindly provided by P. VR-1616 determined by each laboratory, using four separate dilution series from a single vial in the Progen AAV2 Titration ELISA Recombinant Adeno-associated Virus 2 (Progen Biotechnik GMBH, Article number PRATV), against a Reference Standard Stock (rAAV2-RSS) standard curve prepared from a previously titered rAAV2 Adeno-associated virus 2 strain H is propagated in 293 [HEK-293] cells (ATCC CRL-1573). The pAV2 vector has fewer polylinker sites than that of pAV1 (HindIII, ClaI, and one EcoRI site have been deleted). Recombinant Adeno-associated virus 2 is a reference material for gene therapy research. This product is intended to be used for laboratory research use only. 8kb material contains a 2. Human adenovirus 5 (H5) reference standard (ATCC VR-1516). AAV plasmids Helper Plasmid Sep 12, 2024 · Aurnhammer et al. 8kb AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA genome in AAV2 capsid, with a genome titer (EGFP targeting) of 1X10^13 VG/ml. ZERO BIAS - scores, article reviews, protocol conditions and more ATCC's Adenovirus, Adeno-associated virus, and Lentiviral reference materials provide the internationally accepted standards needed for assay validation, vector quantification, and inter-laboratory data harmonization. Many of these hybrid viruses have been well characterized and may be preferred over standard viruses for in vivo applications. mfbjhezcbkqvwxpidghzszghvfaxbpbmgjesyaopgtkvarujuyosmjweghuetuvqrjskjjdst